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. 2016 Oct 14;9(5):411–418. doi: 10.1016/j.tranon.2016.07.006

Figure 1.

Figure 1

Protein expression of MCM2 and MCM3 in small intestinal mucosa and SI-NENs (primaries and metastases).

Immunohistochemistry (A): MCM2 and MCM3 were identified in crypts associated with the proliferative zone (PZ) (inset) which is the site of cells involved in renewing mucosa where the highest mitosis rates are expected. Additional cells were MCM3 positive at the base of crypts; these cells were not stained by MCM2 (red arrows). Dual staining with chromogranin A identified only a few (<2%) normal neuroendocrine cells to be MCM2 or MCM3 positive (yellow arrows). Both primary tumor and metastasis exhibited nuclear expression of MCM2 and MCM3, and expression of both proteins tended to be higher in metastasis in comparison to the primary tumor. This overexpression was also identified by Western blot (B), especially for MCM3, but this was not statistically significant (Kruskal-Wallis, MCM3: P = .06, MCM2: P = .15).

DAPI: nuclei (blue), FITC: target marker (green), Cy5: chromogranin A (red-white arrows), dual-stained cells (yellow). PZ = proliferative zone. Mean ± SD.