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. 2004 Jul 19;32(13):3792–3798. doi: 10.1093/nar/gkh706

Table 1. PNA sequences.

Name Sequence Mass expected/found Tm (°C) Target IRES sequence
P1 GAGTGATCTATGGTGGA 4868/4871 78 26–42
P2 ACGCCATGGCTAGACGC 4743/4748 80 75–91
P3 GTTGATCCAAGAAAGGA 4830/4827 74 191–207
P3–19 GTTGATCCAAGAAAGGACC 5332/5335 83 191–209
P3–21 GTTGATCCAAGAAAGGACCCG 5876/5879 88 191–211
P4 GCGGGGGCACGCCCAAA 4793/4795 89 226–242
P5 TTTCGCGACCCAACACT 4653/4664 81 260–276
P5-scrambled CATCATCGATCCTAGCC 4653/4650 74 Control
P5-sense AGTGTTGGGTCGCGAAA 4853/4850 80 Control
P6–15 ACGAGACCTCCCGGG 4201/4202 83 315–329
P6 TACGAGACCTCCCGGGG 4759/4764 85 314–330
P6–19 CTACGAGACCTCCCGGGGC 5261/5261 88 313–331
P6–21 TCTACGAGACCTCCCGGGGCA 5803/5800 90 312–332
P7 GTGCTCATGGTGCACGG 4805/4810 83 333–349
P9 TGTCGTTCGCGGGCGCA 4781/4780 90 F-luc

PNAs are listed from N- to C-termini. All PNAs contain a C-terminal lysine. Tm values are for hybridization to exactly complementary sequences. F-luc: a sequence within the coding region for firefly luciferase.