Table 1. PNA sequences.
Name | Sequence | Mass expected/found | Tm (°C) | Target IRES sequence |
---|---|---|---|---|
P1 | GAGTGATCTATGGTGGA | 4868/4871 | 78 | 26–42 |
P2 | ACGCCATGGCTAGACGC | 4743/4748 | 80 | 75–91 |
P3 | GTTGATCCAAGAAAGGA | 4830/4827 | 74 | 191–207 |
P3–19 | GTTGATCCAAGAAAGGACC | 5332/5335 | 83 | 191–209 |
P3–21 | GTTGATCCAAGAAAGGACCCG | 5876/5879 | 88 | 191–211 |
P4 | GCGGGGGCACGCCCAAA | 4793/4795 | 89 | 226–242 |
P5 | TTTCGCGACCCAACACT | 4653/4664 | 81 | 260–276 |
P5-scrambled | CATCATCGATCCTAGCC | 4653/4650 | 74 | Control |
P5-sense | AGTGTTGGGTCGCGAAA | 4853/4850 | 80 | Control |
P6–15 | ACGAGACCTCCCGGG | 4201/4202 | 83 | 315–329 |
P6 | TACGAGACCTCCCGGGG | 4759/4764 | 85 | 314–330 |
P6–19 | CTACGAGACCTCCCGGGGC | 5261/5261 | 88 | 313–331 |
P6–21 | TCTACGAGACCTCCCGGGGCA | 5803/5800 | 90 | 312–332 |
P7 | GTGCTCATGGTGCACGG | 4805/4810 | 83 | 333–349 |
P9 | TGTCGTTCGCGGGCGCA | 4781/4780 | 90 | F-luc |
PNAs are listed from N- to C-termini. All PNAs contain a C-terminal lysine. Tm values are for hybridization to exactly complementary sequences. F-luc: a sequence within the coding region for firefly luciferase.