Skip to main content
. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Cornea. 2016 Nov;35(11):1466–1470. doi: 10.1097/ICO.0000000000000954

Figure 2. ECM mRNA and protein expression in miR-29b Psh transfected immortalized Fuchs dystrophy corneal endothelial cells.

Figure 2

RT-PCR (A): Compared to control Psh transfected cells, miR-29b Psh transfected cells showed the following levels of ECM gene expression (mean ± standard deviation): COL1A1: 1.9 (±0.4)%; COL4A1: 7.1 (±1.7)%; LAMC1: 21.5 (±2.7)% (*p < 0.05, n=3 per each condition, bar: standard deviation). Western blot: compared to control Psh transfected cells, miR-29b Psh transfected cells showed the following levels of ECM protein expression (mean ± standard deviation): (B) COL1A1: 4.8 (±3.2)% , (C) COL4A1: 42.5 (±25.0)% , and (D) LAMC1: 44.8 (±3.1)% (*p < 0.05, n = 3 per each condition, bar: standard deviation).