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. 2004 Jul 25;32(13):3898–3903. doi: 10.1093/nar/gkh724

Table 1. Steady-state kinetic parameters of the WT M.SinI and the L214S + Y229H double-mutant enzyme.

DNA substrate	M.SinI (WT)		M.SinI (L214S + Y229H)
	GG^A/_TCC	GG^G/_CCC	GG^A/_TCC	GG^G/_CCC
K_m (nM)	17	116	20.7 ± 0.34	255 ± 15
k_cat (min⁻¹)	0.3	0.014	0.08 ± 0.0048	0.56 ± 0.06
k_cat/K_m (10⁵ M⁻¹ s⁻¹)	2.94	0.02	0.65	0.37