Table 1.
UHPLC-MS diagnostic ions (ESI-negative mode) used for the identification of biomarkers in ASM-, AZA-, and RIB-treated tobacco cells.
| Rt | m/z | Compound name | Diagnostic m/z fragment ions | ASM | AZA | RIB | MJ | SA | ABA |
|---|---|---|---|---|---|---|---|---|---|
| 3.44 | 411.172 | Caffeoylputrescine glycoside (1) | 321.14, 249.12, 179.12, 135.04 | • | • | • | • | ||
| 3.59 | 353.086 | 3-Caffeoylquinic acid (2)* | 191.05, 179.05, 135.04 | • | • | ||||
| 4.05 | 355.092 | Feruloylglycoside I (3) | 193.04 | • | • | ||||
| 4.38 | 353.085 | trans-5-Caffeoylquinic acid (4)* | 191.05, 135.04 | • | • | • | • | ||
| 4.50 | 353.086 | 4-Caffeoylquinic acid (5)* | 191.05, 179.03, 173.04, 135.04 | • | |||||
| 5.33 | 355.091 | Feruloylglycoside II (3) | 193.05 | • | • | ||||
| 5.47 | 353.087 | cis-5-Caffeoylquinic acid (6)* | 191.05, 135.04 | • | • | • | • | ||
| 5.77 | 337.194 | 5-p-Coumaroylquinic acid (7) | 191.03 | • | • | ||||
| 6.05 | 335.071 | Caffeoylshikimic acid (8) | 179.03, 135.04 | • | • | • | |||
| 7.24 | 429.056 | Caffeoylputrescine conjugate (9) | 249.08, 179.00, 135.08 | • | |||||
| 7.38 | 630.116 | tri-Caffeoylspermidine (10) | 468.10 | • | |||||
| 7.65 | 474.181 | Feruloyltyramine glycoside I (11A/B) | 312.14, 178.07 | • | • | • | |||
| 7.79 | 444.162 | Coumaroyltyramine glycoside II (12A/B) | 282.04 | • | |||||
| 7.95 | 504.183 | Feruloyl-3-methoxytyramine glycoside I (13A/B) | 342.13, 178.04 | • | |||||
| 8.28 | 474.172 | Feruloyltyramine glycosides II (11A/B) | 312.11, 178.05 | • | |||||
| 8.30 | 504.182 | Feruloyl-3-methoxytyramine glycoside II (13A/B) | 312.14, 178.07 | • | • | ||||
| 8.36 | 515.161 | 3,4-diCaffeoylquinic acid (14)* | 535.06, 191.05, 179.03, 173.05, 135.04 | • | |||||
| 8.64 | 546.194 | Feruloyl-3-methoxytyramine conjugate I (15A/B) | 342.13, 178.10 | • | • | • | |||
| 8.73 | 515.120 | 4,5-diCaffeoylquinic acid (16)* | 353.03, 191.05, 179.03, 173.04, 135.04 | • | |||||
| 8.86 | 546.196 | Feruloyl-3-methoxytyramine conjugate II (15A/B) | 342.13, 178.10 | • | • |
The common and unique biomarkers in response to the priming agents are shown and compared to the phytohormones salicylic acid (SA), methyljasmonate (MJ), and abscisic acid (ABA) associated with defense responses.
*
Authentic standards were used to validate the identification.