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. 2016 Oct 18;9:100. doi: 10.3389/fnmol.2016.00100

FIGURE 3.

FIGURE 3

Differences in Mechanical Thresholds and Percentage of Paw Withdrawals between spared and non-spared regions in the tibial-SNI and sural-SNI models. (A) Mechanical Thresholds measurements in the ipsilatereal (IL) and contralateral (CL) paws in both the spared and non-spared regions of sural-SNI and tibial-SNI rats. No significant difference was found between the spared vs. non-spared regions in the IL paws for the tibial-SNI, neither for the sural-SNI. Significant different: symbols in black: non-spared-IL vs. non-spared-CL for a given SNI (# tibial-SNI, sural-SNI). Inline graphic(green): spared-IL vs. spared-CL regions in tibial-SNI. Inline graphic(blue): spared-IL vs. spared-CL regions in sural-SNI. Inline graphic(red): spared-IL tibial-SNI vs. spared-IL sural SNI. Inline graphic(purple): non-spared-IL tibial-SNI vs. non-spared-IL sural-SNI. For all the groups n = 6 rats for most days, except for Days 20, 71, and 75 where n = 3 rats, and Day 83 where n = 4 rats. One exception is for tibial-SNI non-spared (CL and IL) that the n = 3 rats on Day 1. (B) Percentage of Paw Withdrawals in the Tibial-area (IL and CL) and Sural-area (IL and CL) in naïve rats. No significant difference was found when comparing between groups at a given day, or within a group between 1–6 Days vs. later days; n = 3 rats. (C) Percentage of Paw Withdrawals in the IL paws of sural-SNI and tibial-SNI. Comparison between groups at a given day: Inline graphic(blue): spared-sural-SNI vs. non-spared sural-SNI. No significant difference was found between spared-tibial-SNI vs. non-spared-tibial-SNI; or between spared-tibial-SNI vs. spared-sural-SNI. Within a group between 1–6 Days vs. later days: + (black): sural-SNI (spared and non-spared); Inline graphic(green) tibial-SNI (spared and non-spared). n = 6 rats (same animals as in A). (D) Percentage of Paw Withdrawals in the CL paws of sural-SNI and tibial-SNI in the CL. For a given day, no significant difference between the sural-SNI-CL and tibial-SNI-CL regions. Within a group between 1–6 Days vs. later days: no significance difference in tibial-SNI; + (black): sural-SNI. (E) Percentage of Paw Withdrawals in the tibial-area (IL and CL) and sural-area (IL and CL) in Sham rats. For a given day, no significant difference was found between the groups. For a given group, significant difference was found between 1–6 Days vs.≥50 Days for three of the groups. The fourth group, the tibial-Area-IL, significant difference was found between 1–6 Days vs.≥65 Days. n = 4 rats; (F) Mechanical Threshold measurements in the IL and CL paws in both Sural and Tibial areas. No significant differences were found; between groups within a given day. Two-way ANOVA Ordinary (A) and Two-way ANOVA Matching Stacked (B–F); followed by Bonferroni’s Multiple comparison (all symbols: p < 0.05). Values are mean ± SEM.