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. 2016 Oct 18;9:100. doi: 10.3389/fnmol.2016.00100

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Copyright © 2016 Norcini, Sideris, Adler, Hernandez, Zhang, Blanck and Recio-Pinto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differences in Mechanical Thresholds and Percentage of Paw Withdrawals between spared and non-spared regions in the tibial-SNI and sural-SNI models. (A) Mechanical Thresholds measurements in the ipsilatereal (IL) and contralateral (CL) paws in both the spared and non-spared regions of sural-SNI and tibial-SNI rats. No significant difference was found between the spared vs. non-spared regions in the IL paws for the tibial-SNI, neither for the sural-SNI. Significant different: symbols in black: non-spared-IL vs. non-spared-CL for a given SNI (# tibial-SNI, ^∗sural-SNI). (green): spared-IL vs. spared-CL regions in tibial-SNI. (blue): spared-IL vs. spared-CL regions in sural-SNI. (red): spared-IL tibial-SNI vs. spared-IL sural SNI. (purple): non-spared-IL tibial-SNI vs. non-spared-IL sural-SNI. For all the groups n = 6 rats for most days, except for Days 20, 71, and 75 where n = 3 rats, and Day 83 where n = 4 rats. One exception is for tibial-SNI non-spared (CL and IL) that the n = 3 rats on Day 1. (B) Percentage of Paw Withdrawals in the Tibial-area (IL and CL) and Sural-area (IL and CL) in naïve rats. No significant difference was found when comparing between groups at a given day, or within a group between 1–6 Days vs. later days; n = 3 rats. (C) Percentage of Paw Withdrawals in the IL paws of sural-SNI and tibial-SNI. Comparison between groups at a given day: (blue): spared-sural-SNI vs. non-spared sural-SNI. No significant difference was found between spared-tibial-SNI vs. non-spared-tibial-SNI; or between spared-tibial-SNI vs. spared-sural-SNI. Within a group between 1–6 Days vs. later days: + (black): sural-SNI (spared and non-spared); (green) tibial-SNI (spared and non-spared). n = 6 rats (same animals as in A). (D) Percentage of Paw Withdrawals in the CL paws of sural-SNI and tibial-SNI in the CL. For a given day, no significant difference between the sural-SNI-CL and tibial-SNI-CL regions. Within a group between 1–6 Days vs. later days: no significance difference in tibial-SNI; + (black): sural-SNI. (E) Percentage of Paw Withdrawals in the tibial-area (IL and CL) and sural-area (IL and CL) in Sham rats. For a given day, no significant difference was found between the groups. For a given group, significant difference was found between 1–6 Days vs.≥50 Days for three of the groups. The fourth group, the tibial-Area-IL, significant difference was found between 1–6 Days vs.≥65 Days. n = 4 rats; (F) Mechanical Threshold measurements in the IL and CL paws in both Sural and Tibial areas. No significant differences were found; between groups within a given day. Two-way ANOVA Ordinary (A) and Two-way ANOVA Matching Stacked (B–F); followed by Bonferroni’s Multiple comparison (all symbols: p < 0.05). Values are mean ± SEM.

Inline graphic — Differences in Mechanical Thresholds and Percentage of Paw Withdrawals between spared and non-spared regions in the tibial-SNI and sural-SNI models. (A) Mechanical Thresholds measurements in the ipsilatereal (IL) and contralateral (CL) paws in both the spared and non-spared regions of sural-SNI and tibial-SNI rats. No significant difference was found between the spared vs. non-spared regions in the IL paws for the tibial-SNI, neither for the sural-SNI. Significant different: symbols in black: non-spared-IL vs. non-spared-CL for a given SNI (# tibial-SNI, ^∗sural-SNI). (green): spared-IL vs. spared-CL regions in tibial-SNI. (blue): spared-IL vs. spared-CL regions in sural-SNI. (red): spared-IL tibial-SNI vs. spared-IL sural SNI. (purple): non-spared-IL tibial-SNI vs. non-spared-IL sural-SNI. For all the groups n = 6 rats for most days, except for Days 20, 71, and 75 where n = 3 rats, and Day 83 where n = 4 rats. One exception is for tibial-SNI non-spared (CL and IL) that the n = 3 rats on Day 1. (B) Percentage of Paw Withdrawals in the Tibial-area (IL and CL) and Sural-area (IL and CL) in naïve rats. No significant difference was found when comparing between groups at a given day, or within a group between 1–6 Days vs. later days; n = 3 rats. (C) Percentage of Paw Withdrawals in the IL paws of sural-SNI and tibial-SNI. Comparison between groups at a given day: (blue): spared-sural-SNI vs. non-spared sural-SNI. No significant difference was found between spared-tibial-SNI vs. non-spared-tibial-SNI; or between spared-tibial-SNI vs. spared-sural-SNI. Within a group between 1–6 Days vs. later days: + (black): sural-SNI (spared and non-spared); (green) tibial-SNI (spared and non-spared). n = 6 rats (same animals as in A). (D) Percentage of Paw Withdrawals in the CL paws of sural-SNI and tibial-SNI in the CL. For a given day, no significant difference between the sural-SNI-CL and tibial-SNI-CL regions. Within a group between 1–6 Days vs. later days: no significance difference in tibial-SNI; + (black): sural-SNI. (E) Percentage of Paw Withdrawals in the tibial-area (IL and CL) and sural-area (IL and CL) in Sham rats. For a given day, no significant difference was found between the groups. For a given group, significant difference was found between 1–6 Days vs.≥50 Days for three of the groups. The fourth group, the tibial-Area-IL, significant difference was found between 1–6 Days vs.≥65 Days. n = 4 rats; (F) Mechanical Threshold measurements in the IL and CL paws in both Sural and Tibial areas. No significant differences were found; between groups within a given day. Two-way ANOVA Ordinary (A) and Two-way ANOVA Matching Stacked (B–F); followed by Bonferroni’s Multiple comparison (all symbols: p < 0.05). Values are mean ± SEM.