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. 2016 Oct 18;9:100. doi: 10.3389/fnmol.2016.00100

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Copyright © 2016 Norcini, Sideris, Adler, Hernandez, Zhang, Blanck and Recio-Pinto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of microglia/macrophages. (A) DRG section immunostained with anti-Iba-1 (green) a microglia/macrophage marker and stained with the nuclear stain Hoechst (blue). Both Iba-1+/NR2B+ cells (circles) and Iba-1+/NR2B- cells (arrows) were detected. The corresponding light picture is also shown. (B) shows the overlap of the color images in (A). DRG sections immunostained with anti-NR2B (red) and stained with Nissl (green) from a control (C) Sural-SNI (D) and Tibial-SNI (E) L3-DRG at Day 23 post-surgery. (A–B) Conventional pictures and (C–E) optical sections (0.86 μm thick, Apotome). Magnification 40× objective. Scale bars: 20 μm.