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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Chem Soc Rev. 2016 Jul 25;45(15):4074–4126. doi: 10.1039/c5cs00287g

Figure 20. Quantitative analysis of GFP expression with TRBO vector.

Figure 20

Fluorescence (in μg GFP/g infiltrated tissue) was measured from N. benthamiana leaves after agrobacterium infiltration (top). Leaves were also imaged under UV light (bottom). The labels in the figure indicate the plasmids used for transformation, and the results indicate the superior expression of protein with TRBO vector compared to previous expression vectors, even with P19 enhancement. pJL-24 is a previous iteration of a 35S promoter-driven TMV-based expression vector that included the expression of all the TMV genes in addition to the gene insert, pJL3:P19 is a plasmid for the expression of the RNA-silencing suppressor protein P19, pJL TRBO-G is a GFP-expressing TRBO vector, and p35S:GFP is a plasmid with GFP expression under the control of a 35S promoter. Reproduced with permission from ref. 450. Copyright 2007 American Society of Plant Biologists.