FIG 7.

Junctional PCR confirms the operonic structure of putative copper-related genes. To detect each of the gene junctions between genes of a putative operon, qualitative reverse transcription-PCR was conducted. PCRs were performed using genomic DNA, RNA, or cDNA as the template. Each reaction mixture was incubated for 5 min at 95°C, followed by 25 cycles of denaturation at 95°C for 30 s, annealing at 54°C for 30 s, and extension at 72°C for 30 s. PCR products were separated in 1% agarose gels and visualized by staining with ethidium bromide. Each PCR was performed three times using biologically independent cDNA samples.