FIG 8.
Expression of putative copper-related genes in response to copper exposure. Expression of putative copper-related genes was measured by qRT-PCR, using primers specific to the initial gene of each operon. RNA was isolated from AB5075 cells exposed for 30 min to the indicated increasing concentrations (micromolar) of CuSO4. Generated cDNA was used as template DNA for qRT-PCR. Relative gene expression was calculated by the 2−ΔΔCT method, using the 16S rRNA gene as the internal reference. Three or four biologically independent replicates of each experiment were performed. Values for each replicate are shown as individual points, and the geometric means are represented by bars. Dashed lines represent significant (2-fold) up- and downregulation.