FIG 7.

Lactobacillus rhamnosus GG supplementation promotes body weight gain and lactobacillus enhancement in chlorpyrifos-exposed Drosophila but does not affect locomotion. Freshly eclosed Drosophila melanogaster flies were treated for 3 days with media containing vehicle, 1 μM CP, LGG, or 1 μM CP plus LGG. (A) Surface-sterilized Drosophila flies were homogenized and drop plated on MRS agar, and the CFU per Drosophila fly were enumerated. (B) Drosophila body weights were determined. (C) Drosophila locomotion was tested in a negative geotaxis assay in which average distance climbed after 3 s (of 15 to 25 flies) in 5 replicate experiments was quantified. Data displayed are means ± standard errors of the means from at least 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.