TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| UA159 | Wild type | |
| ΔcidA mutant | ΔcidA::npKm | 6 |
| ΔcidB mutant | ΔcidB::npKm | 6 |
| ΔcidAB mutant | ΔcidAB::npKm | This study |
| ΔcidA::ΩKm mutant | ΔcidA::ΩKm | 6 |
| ΔlrgA mutant | ΔlrgA::npSp | 6 |
| ΔlrgB mutant | ΔlrgB::npEm | 6 |
| ΔlrgAB mutant | ΔlrgAB::ΩKm | 6 |
| ΔlrgA ΔcidB mutant | ΔlrgA::npSp ΔcidB::npKm | This study |
| ΔlrgB ΔcidB mutant | ΔlrgB::npEm ΔcidB::npKm | This study |
| ccpA-deficient mutant | ΔccpA::ΩKm | 29 |
| vicK-deficient mutant | ΔvicK::npKm | 3 |
| UA159 ΩPldh-cidAB | Pldh-cidAB integrated into the chromosome of UA159 (strain constitutively expressing cidAB) | This study |
| UA159 ΩPldh-lrgAB | Pldh-cidAB integrated into the chromosome of UA159 (strain constitutively expressing lrgAB) | This study |
| 184-cidAB/UA159 | UA159 carrying pIB184::cidAB (cidAB-overexpressing strain) | This study |
| 184-cidA/UA159 | UA159 carrying pIB184::cidB (cidA-overexpressing strain) | This study |
| 184-lrgAB/UA159 | UA159 carrying pIB184::lrgAB (lrgAB-overexpressing strain) | This study |
| 184-lrgA/UA159 | UA159 carrying pIB184::lrgA (lrgA-overexpressing strain) | This study |
| Plasmid | ||
| pIB184 | Shuttle expression plasmid with constitutive P23 promoter, Em | 20 |
a
np, nonpolar; p, polar; Km, kanamycin resistant; Em, erythromycin resistant; Sp, spectinomycin resistant.