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Mutagenicity of oxanine in DNA. (A) Addition of dNTP to O-containing DNA by E.coli polymerase I Klenow Fragment (3′ exo-). Addition of dOTP and individual dNTP by Klenow Fragment was assayed as detailed in Materials and Methods. HEX, fluorophore (Integrated DNA Technologies) and Cntl, substrate control. (B) Extension of primer on O-containing template. The primer extension reactions were performed as detailed in Materials and Methods with indicated species of dNTPs.
