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Cleavage activity of purified Tma endo V on O-containing DNA. The cleavage reactions were performed as detailed in Materials and Methods with 1, 10 and 100 nM Tma endo V protein. Cleavage products were separated from remaining substrate by electrophoresis. M, 36mer marker; N = A, T, G or C; FAM, fluorophore (Integrated DNA Technologies); and -, control reactions without addition of endo V.
