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. 2016 Oct 17;213(11):2473–2488. doi: 10.1084/jem.20151794

Figure 6.

Figure 6.

SIRT1 ablation in VSMCs abolished the preventive effect of CR on AAA formation. (A) Representative images showing the macroscopic features of normal aortas and aneurysms of Sirt1flox/flox;Apoe−/− mice and SVKO;Apoe−/− mice in the indicated groups. Bar, 1 mm. (B) Maximal abdominal aortic diameter of Sirt1flox/flox;Apoe−/− mice and SVKO;Apoe−/− mice in the indicated groups. n = 10–25. (C and D) Incidence of AAAs (C) and survival curve (D) of Sirt1flox/flox;Apoe−/− mice and SVKO;Apoe−/− mice after AngII infusion. n = 19–25. (E) Total aortic weight–to-BW ratio of Sirt1flox/flox;Apoe−/− mice and SVKO;Apoe−/− mice in the indicated groups. n = 10–15. (F and G) Representative images of EVG staining of abdominal aortic sections of Sirt1flox/flox;Apoe−/− and SVKO;Apoe−/− mice (F) and semiquantitative analysis of elastin degradation (G). Bars: (top) 200 µm; (bottom) 100 µm. n = 6–8. All values are shown as the means ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001. P-values were obtained using a Fisher’s exact test (C), by a log-rank (Mantel-Cox) test (D), by one-way ANOVA plus a Bonferroni test (G), or by a Kruskal-Wallis test plus a Dunn’s multiple comparison test (B and E). F/F, flox/flox.