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. 2016 Oct 17;213(11):2249–2257. doi: 10.1084/jem.20151998

Figure 3.

Figure 3.

AhR regulates the homeostasis of NK cells. (A) NK (NK1.1+CD3−) cells in the BM, spleen, and liver of WT mice were assessed for proliferation by BrdU incorporation. (B) Enriched splenic NK cells from AhR+/+ and AhR−/− mice were labeled with CFSE and cultured in the presence of 1,000 U/ml IL-2. CFSE dilution staining in NK cells is shown at day 3. (C) Representative growth curves for liver mononuclear cells (left) or enriched splenic NK cells (middle) isolated and cultured in 1,000 U/ml IL-2 and 200 nM FICZ or vehicle control. (Right) Representative growth curves of enriched splenic NK cells cultured in 100 ng/ml IL-15 and 200 nM FICZ or vehicle control. (D) Expression of KLRG1 on NK cells in IL-2 or IL-15 assessed by flow cytometry on day 7. (E) Expression of Tim-3 on IL-2–cultured liver (left) and splenic (right) NK cells at day 7. (F) Expression of annexin V on IL-2–cultured splenic NK cells at day 7. Circles, AhR+/+ or AhR+/−; triangles, AhR−/−; closed, vehicle control; open, FICZ. Scatter plots are from at least two independent experiments, and n = 3–8 mice per group. All representative data are from at least three independent experiments. Data are shown as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student’s t test for AhR+/+ vs. AhR−/−; paired Student’s t test for vehicle vs. FICZ).