Figure 1.

Identifying IRF7 binding proteins and investigating their roles in IFN-α production. (A) Gen2.2 cells were left untreated or treated with 1 µM CpG A for 6 h, and then the cells were pooled together, lysed, and immunoprecipitated with anti-IRF7 antibody or isotype control. IRF7 binding proteins were analyzed by mass spectrometry. (B) Selected IRF7 binding proteins and the peptides hit in mass spectrometry are listed. (C) IRF7-associated genes were knocked down by using siRNA targeting specific molecules or nonspecific siRNA control (siNS), and the knockdown cells were stimulated with 1 µM CpG A for 20 h. IFN-α production in the supernatants was analyzed by ELISA. Data are representative of two independent experiments and are presented as the mean ± SEM of triplicate wells. *, P < 0.05; **, P < 0.01 by Student’s t test.