Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 17;213(11):2459–2472. doi: 10.1084/jem.20160635

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Cortizas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Compromised proliferation of Ung-deficient B cells expressing AID. (A, left) Representative microscopy pictures of anaphases from CH12F3 cells expressing the HPV16 E6 and E7 oncoproteins and GFP control, Ugi, or Ugi shAID. Total DNA was stained with DAPI (shown in green). Bars, 2 µm. (Right) Mean + SD proportion of anaphases showing chromosome bridges in CH12F3 GFP and CH12F3 Ugi cells from at least three independent experiments. 50 anaphases were analyzed for each cell line per experiment. (B) Cell cycle profile analysis by BrdU incorporation and propidium iodide (PI) staining in the indicated CH12F3 cells 24 h after stimulating CSR. (C) Cell number (left) and CFSE staining (right) were used to evaluate cellular proliferation of dCH12F3 and CH12F3 Ugi cells expressing the indicated shRNAs after stimulation for CSR. Error bars represent mean + SD from at least three independent experiments. shTERT, shRNA against the telomerase reverse transcriptase (TERT).