Fig. S4.

Expression of SAM-TIR-Venus constructs. Embryonic DRG neurons were preincubated with 1 mM NR and then infected with the indicated lentivirus expressing SAM-TIR-Venus (WT or mutant). Shown are representative images of Venus fluorescence to illustrate SAM-TIR-Venus expression as well as bright-field images illustrating cell death upon expression of WT SAM-TIR-Venus and K597E SAM-TIR-Venus. Venus expression of WT and K597E SAM-TIR-Venus is largely undetectable unless DRGs are preincubated with NR. However, SAM-TIR-Venus with E596K or D627K point mutations does not induce toxicity and does express in the presence or absence of NR. (Scale bar, 25 μM.)