Fig. S7.

Characterization of Cer-SARM-Ven FRET sensor in SARM1^−/− DRG axons. (A) Cer-SARM-Ven FRET construct rescues axon degeneration in SARM1^−/− DRGs. SARM1^−/− DRGs were transduced with an empty vector or lentivirus expressing Cer-SARM-Ven. Axons were severed, and axon degeneration was measured 24 h later. Representative images of distal axons are shown with quantification of axon degeneration from three independent experiments. (B) FRET ratio was measured in distal axons from SARM1^−/− at the indicated time after axotomy. Error bars reflect ± SEM (n = 3).