Fig. S8.

SARM1 TIR domain physically associates with the N terminus/SAM domains of SARM1. (A) Diagram of biochemical strategy to identify the complex between the TIR domain and N terminus/SAM domains from SARM1. SARMps is expressed in HEK293T cells in the presence or absence of FKBP-C-TEV. When cells are treated with rapamycin, the reconstituted TEV protease cleaves SARM1 at an engineered TEV protease site upstream of the TIR domain. In cell extracts, the liberated TIR domain is immunoprecipitated via a Cerulean tag at the C terminus. The N-terminal portion of SARM1 is detected in TIR immunoprecipitates by Western immunoblotting (WB) with SARM1 antisera generated against a peptide from the SARM1/SAM2 domain. (B) Full blots from Fig. 7C. Nonspecific bands in blots from GFP immunoprecipitates are noted with an asterisk (*). clv., cleaved; Co-IP, coimmunoprecipitation; f.l., full length.