Skip to main content
. 2016 Sep 26;113(41):11543–11548. doi: 10.1073/pnas.1613307113

Fig. 4.

Fig. 4.

Arid5a-deficient Th1 cells expressed decreased levels of IFN-γ and T-bet in vitro and in vivo. (A) Naive CD4+ T cells from WT and Arid5a-deficient mice were stimulated under Th0 conditions (stimulated with CD3ε and CD28) or Th1 conditions (stimulated with CD3ε, CD28, and IL-12). Forty-eight hours after differentiation, IFN-γ, T-bet, and STAT4 mRNA expression was measured by RT-PCR. (B) Immunoblot analysis of phosphorylated STAT4 and total STAT4 in WT or Arid5a-deficient CD4+ T cells was stimulated for 12 h under Th0 and Th1 cell conditions. (C) Quantitative real-time PCR analysis of IFN-γ, T-bet, and STAT4 mRNA in CD4+ T cells isolated from the spleens of LPS (5 μg/kg)-challenged WT or Arid5a-deficient mice. The data are representative of three independent experiments (A and C). Error bars show the means ± SD (A–D). *P < 0.05; **P < 0.01 (Student’s t test).