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. 2016 Sep 26;113(41):11543–11548. doi: 10.1073/pnas.1613307113

Fig. 6.

Fig. 6.

Arid5a associates with the stem loop-like structure located near the ARE region of T-bet mRNA. (A) Schematic diagram of the luciferase vectors encoding T-bet 3′UTR region (1–701), T-bet 3′UTR region (1–302), or T-bet 3′UTR region (321–701). The black circle shows AU-rich elements (AREs). (B) Luciferase activity of the pGL3 vectors encoding T-bet 3′UTR region (1–701), T-bet 3′UTR region (1–302), or T-bet 3′UTR region (321–701) cotransfected for 48 h with an Arid5a expression vector or empty vector. The data are representative of three independent experiments. Error bars show the means ± SD (B). *P < 0.05; **P < 0.01; N.S., not significant (Student’s t test). (C) Schematic diagram shows the conserved stem loop-like element (616–641) between humans and mice near the ARE region (closed circle) of T-bet 3′UTR mRNA. (D) Diagram of the stem loop structure (616–641) of the T-bet 3′UTR. (E) EMSA evaluating the interaction of recombinant Arid5a protein with 3′ biotinylated nucleotides, as in D.