Fig. S7.
A pbpG mutation partially bypasses the requirement for the spoIIIA operon. Analysis of SigG activity in sporulating cells harboring the indicated mutants in the A–Q complex in the presence of wild-type pbpG (Left) or a pbpG mutant (pbpG ∆R147-K148) (Right). For cells containing wild-type pbpG, the strains used were wild-type (BCR877), ΔAG (BCR878), ΔAH (BCR879), and ΔspoIIIA (∆IIIA) operon-null (BCR990). For the mutant pbpG (mut) allele, the strains used were wild-type (BCR896), ΔAG (BCR959), ΔAH (BCR900), and ΔIIIA (BCR991). Images are PsspB-CFP (Left) and merged with TMA-DPH–stained membranes (Right). The images were scaled identically. (Scale bar, 2 μm.)