Fig. S8.
The pbpG mutation does not suppress the forespore morphology defects of the ∆AH and ∆AG mutants. Representative images of SigG activity and forespore morphology assessed with the membrane dye TMA-DPH in strains harboring wild-type pbpG or a pbpG mutant (pbpG ∆R147-K148) in wild-type cells (A), in cells lacking AH (∆AH) (B), and in cells lacking AG (∆AG) (C). Images are from hour 4 of sporulation. We note that sporulating cells harboring the pbpG mutation exhibit forespore morphology defects that are different from the small and collapsed forespores observed in cells lacking members of the A–Q complex. Yellow arrowheads point to forespores with abnormal morphologies in the pbpG mutant, and red arrowheads point to forespores that failed to thrive and/or collapsed. Images show TMA-DPH–stained membrane (Left) and merged with PsspB-CFP (Right). (Scale bars, 2 μm.)