Table S1.
Strains, plasmids, and oligonucleotides
| Construct | Genotype/description/sequence | Source or reference |
| B. subtilis strains | ||
| PY79 | Prototrophic wild type | (26) |
| BCR267 | spoIIQ::kan | This work |
| BCR776 | spoIIIAG::markerless | |
| BCR859 (AHB1331) | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat | (4) |
| BCR877 | ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat | This work |
| BCR878 | spoIIIAG::markerless, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat | This work |
| BCR879 | spoIIIAH::erm, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat | This work |
| BCR896 | ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, pbpG(∆R147-K148)Ωphleo | This work |
| BCR900 | spoIIIAH::erm, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, pbpG(∆R147-K148)Ωphleo | This work |
| BCR929 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIAH::erm | This work |
| BCR935 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIA::kan | This work |
| BCR946 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIAG::markerless | This work |
| BCR959 | spoIIIAG::markerless, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, pbpG(∆R147-K148)Ωphleo | This work |
| BCR961 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIAH::erm, pbpG(∆R147-K148)Ωphleo | This work |
| BCR964 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIA::kan, pbpG(∆R147-K148)Ωphleo | This work |
| BCR976 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIAG::markerless, pbpG(∆R147-K148)Ωphleo | This work |
| BCR990 | ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, spoIIIA::kan | This work |
| BCR991 | ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, pbpG(∆R147-K148)Ωphleo, spoIIIA::kan | This work |
| BCR992 | spoIIIGΔ1, amyE::PspoIIQ-sigG::spec, ΔcsfB::tet, ywrK::Tn917::amyE::PsspB-cfp(Bs)::cat, pbpG(∆R147-K148)Ωphleo | This work |
| BCR1434 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAG (kan) | This work |
| BCR1435 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAGR209E (kan) | This work |
| BCR1436 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAGK223E (kan) | This work |
| BCR1437 | spoIIIAG::markerless, ycgO::PsspB-RBSopt-gfp (spec) | This work |
| BCR1438 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAG (kan), ycgO::PsspB-RBSopt-gfp (spec) | This work |
| BCR1439 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAGR209E (kan), ycgO::PsspB-RBSopt-gfp (spec) | This work |
| BCR1440 | spoIIIAG::markerless, yhdG::PspoIIIA (2)-spoIIIAGK223E (kan), ycgO::PsspB-RBSopt-gfp (spec) | This work |
| Plasmids | ||
| pCR094 | His6-SUMO-spoIIIAG (51-229, B. subtilis) | This work |
| pCR133 | His6-SUMO-spoIIIAG (90-229, B. subtilis) | This work |
| pCR134 | His6-SUMO-spoIIIAG (66-201, A. longum) | This work |
| pCR137 | His6-SUMO-spoIIIAG (78-212, G. thermodenitrificans) | This work |
| pCR244 | His6-SUMO-spoIIIAGR209E (51-229, B. subtilis) | This work |
| pCR245 | His6-SUMO-spoIIIAGK223E (51-229, B. subtilis) | This work |
| pCR247 | His6-SUMO-spoIIIAGR209A (51-229, B. subtilis) | This work |
| pCR248 | His6-SUMO-spoIIIAGK223A (51-229, B. subtilis) | This work |
| pCR257 | yhdG::PspoIIIA (2)-spoIIIAG (kan) | This work |
| pCR258 | yhdG::PspoIIIA (2)-spoIIIAG R209E (kan) | This work |
| pCR259 | yhdG::PspoIIIA (2)-spoIIIAGK223E (kan) | This work |
| Oligonucleotides | ||
| oCR160 | gctcacagagaacagattggtggttcttcacctgagaaaactgaaaacg | This work |
| oCR161 | tcgacggagctctgctcttctaccttatgaatcctcctttatttttttag | This work |
| oCR302 | gctcacagagaacagattggtggtgctgactcgatcgatgactatgaa | This work |
| oCR433 | cgcggatccttatgaatcctcctttatttttttag | This work |
| oCR486 | gcgctcgagcttttcaagacagaccccgaagt | This work |
| oCR515 | accattatcgaagcggtgacagaggtcctggatgttccaagccac | This work |
| oCR516 | gtggcttggaacatccaggacctctgtcaccgcttcgataatggt | This work |
| oCR517 | caccgggttgcggttgcccctgaaaaaataaaggaggattca | This work |
| oCR518 | tgaatcctcctttattttttcaggggcaaccgcaacccggtg | This work |
| oCR521 | accattatcgaagcggtgacagcggtcctggatgttccaagccac | This work |
| oCR522 | gtggcttggaacatccaggaccgctgtcaccgcttcgataatggt | This work |
| oCR523 | caccgggttgcggttgcccctgcaaaaataaaggaggattca | This work |
| oCR524 | tgaatcctcctttatttttgcaggggcaaccgcaacccggtg | This work |