(A) ECs transfected with silencing RNA against LATS1 (siLATS1) or siCtrl were subjected to 24-h LS or kept under static condition, followed by the Western blot analysis of P-YAP, YAP, LATS1, and GAPDH. (B) ECs transfected with the constitutively active form of RhoA (V14-RhoA) or a Rho inhibitor (C3 exoenzyme) followed by 24-h LS or OS, were subjected to the Western blot analysis of P-YAP, YAP, TAZ, and GAPDH. Results are representative images from three independent experiments with similar trend.