Transcriptome analysis reveals that laquinimod induces genes in the AhR pathway. (A) This heatmap was generated using the patterns of expression of splenocyte samples from EAE mice at day 6 postinduction. It shows the patterns of expression of 88 genes with higher levels of expression in EAE mice treated with laquinimod (green, n = 6) than in EAE mice treated with vehicle (orange, n = 6). (B) This heatmap was generated using the patterns of expression of splenocyte samples from naive mice at 6 d postinduction. It shows the patterns of expression of 45 genes with higher levels of expression in naive mice treated with laquinimod (red, n = 6) than in naive mice treated with vehicle (blue, n = 5). For both A and B, the filters used to define statistical significance were a fold-change > 2 and an FDR < 0.01. Rows were ordered from highest to lowest average fold-change. (C) In vivo induction of hepatic Cyp1a mRNA following 5-d treatment with 25 mg/kg laquinimod in C57BL/6 mice. This graph is taken from a representative experiment, which has been repeated at least four times.