Fig. 1.

AAL binding to nuclei of T. gondii is stage- and species-specific. (A) Schematic of the T. gondii life cycle. (B) AAL binds to nuclei of tachyzoites, bradyzoites, and sporozoites but not oocysts. (C) The Apicomplexa phylogenetic tree. (D) AAL binds to closely related organisms (H. hammondi and N. caninum) but not to more distantly related apicomplexans (S. neurona, E. tenella, and P. falciparum). Binding to sporozoites is shown for all species, with the exception of P. falciparum for which a trophozoite is shown. IMC3 marks the inner membrane complex, centrin marks the centrosome, and epichromatin marks the nuclear periphery. Griffonia simplicifolia lectin II (GSLII) labels the short P. falciparum N-glycans. All images were taken by deconvolution fluorescence microscopy. (Scale bars: 2 μm.)