Fig. 2.

AAL specifically binds to O-glycans on T. gondii glycoproteins. (A) AAL binding to extracellular tachyzoites by flow cytometry shows inhibition by preincubation with αMeFuc. (B) β-Elimination, but not PNGase F treatment, inhibits AAL binding to tachyzoite cell lysates. Tubulin is shown as a loading control. (C) Protease treatments show that AAL binds exclusively to glycoproteins. C, chymotrypsin; PK, proteinase K; T, trypsin; U, untreated. (D) Disruption of GDP-fucose synthesis using CRISPR/Cas9 eliminates AAL binding by deconvolution fluorescence microscopy. (Scale bars: 4 μm.)