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Costaining of SRD-YFP, NLS-YFP, and RH with additional nuclear markers. (A and B) SRD-YFP is excluded from the nucleolus (A), whereas NLS-YFP is enriched in this subnuclear compartment (B). (C) AAL binds to nuclear subdomains distinct from euchromatin, as defined by antibodies against two histone modifications associated transcriptional activation: H3K9Ac (Upper) and H3K4Me3 (Lower). (Scale bars: 2 μm.) All images were taken on a deconvolving fluorescence microscope.
