Fig. 1.

B7-H1 restricts spontaneous CNS autoimmunity and prevents T-cell infiltration into the brain of sick OSE mice. (A) Percentages of OSE^WT and OSE^KO mice that spontaneously developed EAE symptoms over a period of 12 wk after birth. Forty-five OSE^WT and 57 OSE^KO mice were analyzed. (B) Survival of 246 OSE^WT and 216 OSE^KO mice was monitored over a period of 140 d after birth. Mice were recorded as dead when they reached the clinical score 5.5 or higher; mice eliminated for reasons unrelated to disease course (all with score below 5.5) were censored (indicated by vertical ticks). (C) Clinical scores of 18 OSE^WT and 18 OSE^KO mice until 70 d of age. (D–F) Immunohistochemical analysis of T-cell infiltration in different CNS regions of 12 OSE^WT and 10 OSE^KO mice (score-matched, mean clinical score 5.75). (D) Representative images of T-cell infiltrates in the spinal cord, corpus callosum, and cerebellum, as indicated. (E) Quantification of the extent of inflammation and T-cell infiltration in the spinal cord parenchyma and leptomeninges, as indicated (Top and Middle); semiquantitative scores of T-cell infiltration in the cerebellum and brainstem, and in the cerebrum (Bottom). (F) Representative images of T-cell infiltration in different brain areas of OSE^WT and OSE^KO mice. (D and F) T cells are indicated by arrowheads. The asterisk in F indicates a postcapillary venule. (Scale bars: D, spinal cord, 200 μm; corpus callosum, 100 μm; cerebellum, 200 μm; F, 100 μm.) (E) Each data point represents one mouse. (A and B) Log-rank (Mantel–Cox) test. (C) Two-way ANOVA with Bonferroni correction for multiple comparisons. (E) Unpaired, two-tailed Student’s t test. *P < 0.05; ***P < 0.001; ns, not significant.