Fig. 2.

B7-H1 limits T-cell infiltration in the cerebellum and brainstem of OSE mice at disease onset. (A) Frequencies of MOG-specific CD4⁺ T cells (percent of CD3⁺CD4⁺) in the lymph nodes, spleen, and brain, as determined by flow cytometry. (B) Absolute numbers of CD4⁺ T cells in different CNS regions of OSE^WT and OSE^KO mice at disease onset (mean age 28 d), as determined by flow cytometry and using cell-counting beads. (C) Absolute numbers of immune cells (as indicated) in the cerebellum and brainstem of OSE^WT and OSE^KO mice at disease onset (mean age 32 d), determined by flow cytometry and using cell-counting beads. Each data point represents one mouse. Unpaired, two-tailed Student’s t test. *P < 0.05; **P < 0.01; ns, not significant.