Skip to main content
. 2016 Sep 26;113(41):E6182–E6191. doi: 10.1073/pnas.1601350113

Fig. S3.

Fig. S3.

Effector molecule production per cell by MOG-specific CD4+ T cells is not limited by B7-H1 in the periphery and brain of sick OSE mice. (A) Gating strategy for flow cytometric analysis of lymph nodes and brains of score-matched OSE mice (mean clinical score 4). Examples of GM-CSF production in the lymph nodes (Top) and GzmB production in the brain (Bottom) by MOG-specific CD4+ T cells are shown. (B–E) Quantification of effector molecule production after 4 h of ex vivo restimulation with PMA, ionomycin, and Golgi Plug. (B and C) Production of IFN-γ, GzmB, IL-17A, GM-CSF, TNF-α, and IL-2 in the lymph nodes expressed as percent of MOG-specific CD4+ T cells (B) and MFI (C). (D and E) Production of IFN-γ and GzmB in the brain, expressed as percent of MOG-specific CD4+ T cells (D) and MFI (E). Each data point represents one mouse. Data show mean ± SEM. Unpaired, two-tailed Student’s t test. *P < 0.05; ns, not significant.