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. 2016 Sep 26;113(41):11597–11602. doi: 10.1073/pnas.1603119113

Table S6.

Genes and their respective oligo sequences (qPCR)

Gene Full name Alias Sequence forward oligo Sequence reverse oligo
Mprip Myosin phosphatase Rho interacting protein CTGACGCAGGCAAAACCC GATGAAGAATCGTCGCTGCC
Gapdh Glyceraldehyde-3-phosphate dehydrogenase AAAAGGGTCATCATCTCCGC ATTTCTCGTGGTTCACACCC
Rps27a Ribosomal protein S27a CAGAGGCTGATCTTTGCTGG GTCTCAACACCAGATGAAGGG
Fos FBJ osteosarcoma oncogene cFOS TCCTACTACCATTCCCCAGC ATAAAGTTGGCACTAGAGACGG
Hspa1a Heat shock protein 1A HSP70 CCTGAACAAGAGCATCAACCC ACGTTCTCCGACTTGTCCC
Hmox1 Heme oxygenase 1 Ho-1 TCAGGTGTCCAGAGAAGGC TGGGTTCTGCTTGTTGCG
Syvn1 Synovial apoptosis inhibitor 1, synoviolin Hrd-1 ACACATTCCCACTCTTTGCC GTACAGTGTGTTCATGTTGCG
Hspa4l Heat shock protein 4 like Osp94 TGGAAATGCAGCTAAGAGCC CAAATGATCGCCCATGAAGC
Gfap Glial fibrillary acidic protein AAGAGACAGAGGAGTGGTATCG CGATAGTCGTTAGCTTCGTGC
Rbfox3 RNA binding protein, fox-1 homolog (C. elegans) 3 NeuN GTAGAGGGACGGAAAATTGAGG ATAGACTGTTCCTACCACAGGG

All oligos were designed to work with 50 mM monovalent cations, 500 nM oligo, 3 mM Mg2+, and 0.8 mM dNTPs at a Tm of 60 °C.