Fig. S3.

PFN1^C71G and PFN1^WT mice express the transgene in spinal cord and in motor neurons at similar levels. (A) Both PFN1^C71G and PFN1^WT mice expressed the transgene broadly in the gray matter of the spinal cord as shown by V5 staining in the lumbar area. The cervical spinal cord shows the same pattern. (B) Spinal cords from PFN1^C71G and PFN1^WT mice were sectioned and doubly stained for V5 and ChAT in parallel. Images were taken using confocal microscopy using the same exposure settings. The fluorescence intensity in individual cells was quantified (unfilled symbols) and averaged from each animal. The averages of individual animals were averaged and plotted as the average of the genotype (filled symbols with error bars, which represent SD). Symbols arranged in one vertical line represent values from cells in one animal.