Fig. 2.

Deficiency of ERdj5 suppresses Ca²⁺ uptake into the ER through SERCA2b. (A and B) Cells were loaded with Mag-Fura-2 to estimate [Ca²⁺]_ER. Semipermeabilized MEFs were treated with EGTA for 20 min to remove Ca²⁺. After depletion of Ca²⁺, ATP and Ca²⁺ were added to stimulate SERCA2 pump functions. Mag-Fura-2 fluorescence was measured as [Ca²⁺]_ER. The quantifications of each Ca²⁺ uptake are as shown in B. (C and D) After 1 µM thapsigargin treatment, [Ca²⁺]i was measured using Mag-Fura-2. Peak amplitudes are shown as a bar graph in D.