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. 2016 Sep 23;113(41):11621–11626. doi: 10.1073/pnas.1610358113

Fig. 5.

Fig. 5.

A loss of endodermal signaling results in changes in auxin distribution and root gravitropism. (A) Seedlings were grown for 5 d on MS medium before transfer for 3 d to estradiol containing medium. (B) Roots from A were transferred back to MS medium and allowed to grow for 3 d during which time normal growth was recovered; the white bars mark the position of the root tip at the time of transfer to estradiol containing medium; the yellow bar marks the position of the root tip at the time of transfer to medium without estradiol. (C–F) Expression of DR5:erGFP in vertically grown roots with the EN7:icals3m transgene. DR5:erGFP in (C) uninduced and (D) induced roots. (D) Arrows note the expression of DR5:erGFP in the lateral root cap after a 2-d block to endodermal signaling. Vertically grown (E) uninduced and (F) induced EN7:icals3m roots expressing the DR5:erGFP marker were gravistimulated (turned 90°). Yellow arrow points to the expression of DR5:erGFP in the downward flank of the root meristem of the uninduced root following gravistimulation. (F) There is no change in DR5:erGFP expression in the induced root in response to gravistimulation.