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. 2016 Sep 28;113(41):E6199–E6208. doi: 10.1073/pnas.1608245113

Fig. 3.

Fig. 3.

Effects of dual p5RHH-p65/p100 siRNA NP treatment on the severity of cartilage damage and reactive synovitis. Mouse knees were subjected to compression injury at 6 N and injected i.a. with 0.1 μg of p65/p100 siRNA combined or scrambled (scram) siRNA NP immediately and at 48 h postinjury. (A) On day 5, knees were processed and examined for TUNEL staining (green) in the impact area (rectangle). The number of TUNEL+ cells was enumerated across the entire impact area (spanning 50- × 5-μm slides) and expressed as mean cell number per section. Comparison of the length of cartilage lesion (based on loss of Safranin O staining between yellow demarcation lines) and reactive synovitis (yellow line) in p5RHH-scram siRNA NP-treated (B) and in p5RHH-p65/p100 siRNA NP-treated (C) knees. Values represent mean ± SEM. n = 4 mice per treatment group. COL2 (red), type II collagen; F, femur; M, meniscus; T, tibia; Tx, treatment. DAPI (blue) stains nuclei. [Scale bars, 100 μm (A) and 250 μm (B and C).] *P < 0.05; **P < 0.01; n.s., not significant.