Fig. 4.

Effect of individual p5RHH-p65 and p5RHH-p100 siRNA NP treatment. Mouse knees were subjected to compression injury at 6 N and left untreated (No Tx) or injected i.a. with 0.05 μg of p5RHH-p65 or p100 siRNA NP immediately and at 48 h postinjury. (A) On day 5, knees were processed and examined for P-p65 and P-p100 in the boxed area by confocal microscopy. (B) MFI of P-p65 (Left) and P-p100 (Right) expression level per chondrocyte was analyzed from z-stack confocal images. (C) The number of TUNEL⁺ cells, length of cartilage lesions (based on loss of Safranin O staining), and reactive synovitis in the different treatment groups. Values represent mean ± SEM; n = 4 mice per treatment group. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant. (Scale bars, 100 μm.)