Skip to main content
. 2016 Sep 28;113(41):E6199–E6208. doi: 10.1073/pnas.1608245113

Fig. 6.

Fig. 6.

Effect of p5RHH-p65 siRNA NP on AMPK/mTOR/β-catenin activity. Mice were subjected to compression injury at 6 N and injected i.a. immediately with 0.1 μg of p5RHH-p65 siRNA NPs or p5RHH-scrambled (scram) siRNA NPs, and knees were harvested at 24 h. (A) Impact injury up-regulated mTOR activity and phosphorylation of S6. (B) AMPK is constitutively activated (left knee); mechanical loading led to profound down-regulation of AMPK activity (right knee). p5RHH-p65 siRNA NP significantly enhanced AMPK phosphorylation. Phospho-S6 and phospho-AMPK intensity in the boxed areas was obtained from z-stack confocal images. (C) Impact injury augmented β-catenin level leading to its nuclear translocation whereas p5RHH-p65 siRNA NP administration suppressed its level. The number of chondrocytes with nuclear β-catenin was enumerated across the entire impact area. Values represent mean ± SEM; n = 3 mice per treatment (Tx) group. COL2 (red), type II collagen; F, femur; M, meniscus; S, synovium; T, tibia. DAPI (blue) stains nuclei. (Scale bars, 100 μm.) *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant.