Fig. S3.

p5RHH-p65/p100 siRNA NP effect on synovium. Mouse knees were subjected to compression injury at 6 N and injected i.a. with 0.1 μg of p5RHH-p65/p100 siRNA combined or p5RHH-scrambled (scram) siRNA NP immediately and at 48 h postinjury. On day 5, knees were processed and examined for P-p65 and P-p100 in the synovium (rectangle). No NF-κB activity was observed in the left, noninjured knee. In contrast, there was significant increase in p65/p100 phosphorylation (green) in the synovium (rectangle) postinjury, in addition to the increased NF-κB activity observed in the cartilage in the right knee. p5RHH-p65/p100 siRNA NP administration suppressed NF-κB activity in the synovium whereas p5RHH-scram siRNA NP did not. COL2 (red), type II collagen; F, femur; M, meniscus. DAPI (blue) stains nuclei. (Scale bars, 200 μm.)