Fig. S5.

Effect of p5RHH-p65 NP on AMPK expression and activity. Mice were subjected to compression injury at 6 N and left untreated or injected i.a. immediately with 0.1 μg of p5RHH-p65 siRNA NPs or p5RHH-scrambled (scram) siRNA NPs; knees were harvested at 24 h and examined for phospho-ACC (P-ACC), a classical target of AMPK (A) or total AMPK expression (B). Fluorescent intensity of P-ACC and total AMPK were examined in the boxed areas by confocal microscopy. Values represent mean fluorescence ± SEM; n = 3 mice per treatment (Tx) group. COL2 (red), type II collagen; F, femur; M, meniscus; S, synovium; T, tibia. DAPI (blue) stains nuclei. (Scale bars, 100 μm.) *P < 0.05; **P < 0.01; n.s., not significant.