Fig. S6.

Alterations in lung myeloid cell composition induced by immunization and chemotherapy. (A) ViM cell numbers in the lungs from individual mice at 1 wk after the first, second, or third dose of weekly vaccination expressed as the absolute number (Left) or as the fraction in total lung leukocytes (Right). (B) Fractions of each lung macrophage population in total lung leukocytes from mice treated as indicated (n = 7–10 per group). Lungs were harvested at 4 wk after the third dose of vaccination and 2 d after the third dose of CY. (C) Cell numbers for each macrophage population or neutrophils in the lung of immunized mice over repeated doses of CY treatment. Before lung harvest at 4 wk after vaccination, mice were given three doses of CY on days −6, −4, and −2 of harvest; two doses on days −4 and −2 of harvest; a single dose on day −2 of harvest; or no dose (n = 5 per group). Each symbol represents one mouse, and error bars represent median with interquartile ranges. *P < 0.05, **P < 0.01, and ***P < 0.001 by the Kruskal–Wallis test, followed by Dunn’s multiple comparisons test. Comparisons were made between untreated mice versus the others in A and C, whereas in B, comparisons were made between Untreat vs. CY, Untreat vs. Vac, and Vac vs. Vac+CY.