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. 2016 Sep 26;113(41):11579–11584. doi: 10.1073/pnas.1608383113

Fig. 3.

Fig. 3.

Binding of MuV-HN proteins to sialyl glycans. (A) Binding of purified MuV-HN protein to six branched/unbranched α2,3- or α2,6-linked sialyl glycans. G1, NeuAcα2,3Galβ1,4GlcNAc-BSA; G2, NeuAcα2,3Galβ1,4GlcNAc β1,2(NeuAcα2,3Galβ1,4GlcNAcβ1,4)Manα1,3(NeuAcα2,3Galβ1,4GlcNAcβ1,2Manα1,6)Manβ1,4GlcNAcβ1,4GlcNAc-BSA; G3, NeuAcα2,3Galβ1,4GlcNAcβ1,2(NeuAcα2,3Galβ1,4GlcNAcβ1,4)Manα1,3(NeuAcα2,3Galβ1,4GlcNAcβ1,2 (NeuAcα2,3Galβ1,4GlcNAcβ1,6)Manα1,6)Manβ1,4GlcNAcβ1,4GlcNAc-BSA; G4, NeuAcα2,6Galβ1,4GlcNAc-BSA; G5, NeuAcα2,6Galβ1,4GlcNAcβ1,2Manα1,3(NeuAcα2,6Galβ1,4GlcNAcβ1,2Manα1,6)Manβ1,4GlcNAcβ1,4GlcNAc-BSA; G6, NeuAcα2,6Galβ1,4GlcNAcβ1,2(NeuAcα2,6Galβ1,4GlcNAcβ1,4)Manα1,3 (NeuAcα2,6Galβ1,4GlcNAcβ1,2Manα1,6)Manβ1,4GlcNAcβ1,4GlcNAc-BSA. The glycans are attached onto BSA. (B) Binding of purified MuV-HN proteins, WT or Y369A, to the trisaccharides NeuAcα2,3Galβ1,4GlcNAc-polyglutamic acid (Left) and NeuAcα2,3Galβ1,4Glc-BSA (Right). Measles virus H protein served as a negative control. Data are the mean ± SD of three samples. N.D., not detected. Data shown in this figure are representative of three independently performed experiments.