Table 4. Total cost estimation for the identification of the 30 individuals of the five species considered (n = number of individuals of each species) found in the sampling stations, based on the time required for the analysis (at left) and consumables/external sequencing (right).

Spanish salaries for laboratory technicians were taken from the official Resolution 2,000 BOE 49 of 26 of February of 2015.

		Diagnosis time (min labour)				Consumables & external analysis
	n	Visual	DNA barcoding			Visual	DNA barcoding
			Tissue sampling	DNA extraction	PCR preparation
Diogenichthys atlanticus	3	15 min × 3 = 45	2 min × 3 = 6 min	15 min	30 min	Fixative	Extraction kit
Hygophum hygomii	22	10 min × 22 = 220	2 min × 22 = 44 min				PCR products
Lampanyctus alatus	1	15 min × 1 = 15	2 min × 1 = 2 min				Sequencing & BLAST
Lampanyctus pusillus	3	15 min × 3 = 45	2 min × 3 = 6 min
Myctophum asperum	1	10 min × 1 = 10	2 min × 1 = 2 min
Total time		335 min	105 min
Estimated cost		60.5€		18.97€		1€×30=30€	5€×30=150€