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. 2016 Oct 13;4:e2574. doi: 10.7717/peerj.2574

Figure 1. EOD-FR induction of eDR5::LUC luminescence.

Figure 1

(A–C) Mean luminescence of 5-day-old seedlings (A), 3-week-old juveniles (B), or 3-week-old juveniles in the presence of NPA (C) moved to darkness (solid black line) or treated with a 30 minute EOD-FR pulse prior to transfer to darkness (dashed red line). Dotted lines indicate SEM. Time 0 indicates the beginning of the EOD-FR treatment. n = 4 − 11 plants for each treatment. Representative plots for one of three independent experiments are shown. (D–F) False-color images of eDR5::LUC plants. Representative DMSO treated plant 40 (D) or 240 (E) minutes after EOD-FR pulse showing increase in petiole luminescence after treatment. (F) NPA treated plants 240 minutes after EOD-FR do not have observable petiole luminescence but show increased luminescence in the leaves and apices. (G) Mean luminescence of 3-week-old juveniles treated with DMSO (compare with (C)). For growth conditions see “seedling stage EOD-FR analysis” (A) and “analysis of juvenile plants under EOD-FR” (B-G) in Materials and Methods.