Table 2. List of candidate enzymes involved in the luciferin metabolic pathway.
A total of 11 candidate genes involved in the luciferin metabolic pathway were identified in the L. aquatilis transcriptome data. An elongation factor 1-alpha was also identified to be used as a control in the RT-PCR experiment.
| EC number | Protein name | Transcript ID | FPKM | Protein ID | Functional description | Accession | Database | Amino acid sequence identity (%) | E-value | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
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| NR | KEGG | Uniprot | |||||||||
| EC: 3.2.1.21 | B-glucosidases | c14185_g1_i1 | 10.85 | c14185_g1_i1| m.15351 | Aryl-phospho-beta-D-glucosidase BglA [Bacillus subtilis (strain 168)] | Q17PP1 | ✓a | ✓ | 44.95 | 2E–58 | |
| c11559_g1_i1 | 1.3 | c11559_g1_i1| m.9194 | Cytosolic beta-glucosidase [Cavia porcellus] | P97265 | ✓a | ✓ | 41.99 | 9E–99 | |||
| EC:1.14.18.1 | Phenol oxidases | c14353_g1_i1 | 230.43 | c14353_g1_i1| m.15909 | Phenoloxidase 2 [Drosophila melanogaster] | Q9V521 | ✓a | ✓ | 57.37 | 0 | |
| c14376_g1_i1 | 320.14 | c14376_g1_i1| m.15966 | Phenoloxidase 2 [Drosophila melanogaster] | Q9V521 | ✓ | ✓ | 62.26 | 0 | |||
| EC: 1.13.12.7 | Luciferases | c10041_g1_i1 | 112.72 | c10041_g1_i1| m.6619 | Luciferase [Luciola lateralis] | Q01158.1 | ✓ | ✓ | 83.67 | 0 | |
| c13054_g1_i1 | 65.83 | c13054_g1_i1| m.12283 | Firefly luciferase [Luciola cruciata] | BAJ41368.1 | ✓ | ✓ | 79.04 | 0 | |||
| EC: 3.1.2.20 | Thioesterases | c9513_g1_i1 | 9.32 | c9513_g1_i1| m.5804 | Acyl-coenzyme A thioesterase 13 [Saimiri boliviensis boliviensis] | XP_003927347.1 | ✓ | ✓a | 87.06 | 0 | |
| c13177_g1_i1 | 9.7 | c13177_g1_i1| m.12638 | Acyl-coenzyme A thioesterase 10, mitochondrial [Tribolium castaneum] | XP_973760.2 | ✓ | ✓a | 60.86 | 7E–179 | |||
| N/A | Luciferin regenerating enzyme (LRE) | c10156_g1_i1 | 55.04 | c10156_g1_i1| m.6798 | Luciferin regenerating enzyme [Lampyris turkestanicus] | ADK55065.1 | ✓ | ✓a | 78.18 | 0 | |
| c12106_g1_i1 | 24.61 | c12106_g1_i1| m.10324 | Luciferin-regenerating enzyme [Luciola cruciata] | BAB85479.1 | ✓ | ✓a | 74.43 | 2E–170 | |||
| c8279_g1_i1 | 0.68 | c8279_g1_i1| m.4268 | Luciferin-regenerating enzyme [Luciola cruciata] | BAB85479.1 | ✓ | ✓a | 56.11 | 4E–43 | |||
| N/A | Elongation factor 1-alpha | c11516_g1_i1 | 4.3 | c11516_g1_i1| m.9114 | Elongation factor 1-alpha [Tribolium castaneum] | XP_966355.1 | ✓ | ✓ | ✓ | 94.24 | 0 |
Notes.
a
Predicted gene name in the database differs from what is presented in the subject column (File S2).