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. Author manuscript; available in PMC: 2016 Oct 18.
Published in final edited form as: ACS Chem Biol. 2016 Jun 27;11(8):2091–2100. doi: 10.1021/acschembio.6b00366

Figure 3.

Figure 3

Chemical and genomic methods for analyzing RNA structure. (A) Chemical modification by RNA structure readers DMS and SHAPE. (B) Schematic for analysis of adduct formation through RNA structure measurements. The experimenter can either analyze adducts through denaturing gel electrophoresis, or more recently, RNA sequencing. (C) SHAPE analysis of RoX RNA. A comparison between RNA structure probing and conservation suggests the lower SHAPE reactive sites (double stranded) are highly conserved and thus are important for function (Figure adapted from ref 77. Copyright Elsevier 2013).