Table 1. Identified putative Talitrus saltator circadian protein-encoding transcripts.

Clock gene query protein	Transcriptome search sequence Accession No. (all D. melanogaster)	Talitrus transcript/protein identifications
		Transcript			Protein
		Trinity contig ID	Lengtha	RACE	Name	CDS	Lengthb
Core clock proteins
CRY2	n/ac	comp100937_c0_seq1c comp102609_c0_seq3c	1,843	5′+ 3′	Tal-CRY2	Full	565
CLOCK	AAC62234	comp100688_c1_seq1	5,723	3′d	Tal-CLK	Partial	1,907
PERIOD	AAF45804	comp102279_c0_seq7	8,001		Tal-PER	Full	1,557
TIMELESS	AAC46920	comp849619_c0_seq1	1,209		Tal-TIM	Partial	402
CYCLE	AAF49107	comp12103_c0_seq1	1,807e		Tal-BMAL1	Full	602
		comp939723_c0_seq1
Clock-associated proteins
PDH	n/af	comp92607_c0_seq2	2,471g	5′+ 3′d	Tal-PDH I	Full	129
		comp97165_c0_seq3	3,392		Tal-PDH II	Full	89
CASEIN KINASE 2 α	AAN11415	comp102480_c0_seq1	5,147		Tal-CK2 α	Full	353
CASEIN KINASE 2 β	AAF48093	comp99101_c0_seq3	1,567		Tal-CK2 β	Full	220
CLOCKWORK ORANGE	AAF54527	comp1009591_c0_seq1	503		Tal-CWO	Partial	167
DOUBLETIME (or CK1 ε)	AAF57110	comp87763_c0_seq1	1,092		Tal-DBT	Full	310
PDP1 ε	AAF04509	comp98345_c0_seq1	3,423		Tal-PDP1 ε	Full	508
PP1	CAA39820	comp97405_c0_seq1	1,725		Tal-PP1	Full	357
PP2A –subunit MICROTUBULE STAR	AAF52567	comp98380_c0_seq1	2,981		Tal-MTS	Full	309
PP2A –subunit WIDERBORST	AAF56720	comp102157_c1_seq1	2,474		Tal-WBT	Full	458
PP2A –subunit TWINS	AAF54498	comp99704_c0_seq3	1,633		Tal-TWS	Full	445
SHAGGY	AAN09084	comp99811_c0_seq7	4,413		Tal-SGG	Full	418
SUPERNUMERARY LIMBS	AAF55853	comp98870_c0_seq1	2,121		Tal-SLIMB	Full	588
VRILLE	AAF52237	comp100474_c0_seq6	3,949		Tal-VRI	Full	509
EBONY	AAF55870	comp99283_c0_seq2	4,380		Tal-EBONY	Full	974
RORA	NP_788301	comp99654_c0_seq3	2,217		Tal-RORA	Partial	599
REVERB	NP_730321	comp101252_c0_seq2	5,385		Tal-REVERB	Full	1,110
SIRT1	NP_477351	comp101818_c1_seq1	4,033		Tal-SIRT1	Partial	955
SIRT2	NP_650880	comp97450_c0_seq4	2,275		Tal-SIRT2	Partial	376
SIRT4	NP_572241	comp92313_c0_seq2	2,977		Tal-SIRT4	Partial	354
SIRT6	NP_649990	comp69157_c0_seq1	1,209		Tal-SIRT6	Partial	402
SIRT7	NP_651664	comp95761_c0_seq1	5,180		Tal-SIRT7	Partial	948
JETLAG	NP_608880	comp100423_c0_seq4	2,454		Tal-JET	Partial	458

Notes.

^alength in nucleotides.

^blength in amino acids.

^cHomology cloning sequence used to search transcriptome. Trinity contigs likely from misassembly, other gene details stated taken from homology cloning and RACE PCR.

^dTrinity contig extended 3^′ sequence past RACE-derived sequence, other gene details stated taken from contig sequence.

^eOverlapping contigs combined.

^fUca pugilator octadecapeptide β-PDH consensus sequence used.

^gIncludes combined contig sequence and overlapping 5^′ RACE sequence.